Multi-omics: analysis of small RNAs and chromatin structure at the level of the single individual

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Understanding the interactions between living organisms and the environment now involves combining and integrating different “omics” approaches that may include the analysis of the structure of the chromatin, the epigenome and the temporal fluctuations of gene expression according to the environmental cues received. These analyzes aim to understand the notions of plasticity which ultimately represent the basis of the adaptability of organisms. On a larger scale, and like the "connectome" currently describing neuronal interaction networks, a new concept could be defined, combining a multi-scale quantitative approach specific to functional biology (of the -omics data) and a predictive ecosystem approach (focused on the individual within his ecosystem in the face of his changing environment). For contemporary epigenetics, i.e. the study of heritable changes in gene function without changes in DNA sequence, the "function" of a gene does not only encompass its ability to encode and produce RNA, but also the retroaction of RNA on the structure of chromatin in “cis” and “trans”.

 

In the thematic school, the techniques for studying chromosomal (ATAC seq) and extra-chromosomal carriers of epigenetic information (small RNA-Seq) will be taught.

 

Total number of expected participants: 30.

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